Cd classification

Author: Prof. Dr. med. Peter Altmeyer

All authors of this article

Last updated on: 29.10.2020

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Synonym(s)

CD Classification; CD Nomenclature; cluster of differentiation

Definition
This section has been translated automatically.

The term Cluster of Differentiation, abbreviation "CD", refers to groups of cellular surface features that have been ordered according to biochemical or functional criteria. The CD classification enables a comprehensible transparency in immunohistological and immunological diagnostics. Currently, 371 cluster molecules are defined and assigned. The protein name is not always identical with the gene name (e.g. CD4- clone 1F6; CD8 - clone DK25).

The CD molecules are mainly membrane-bound glycoproteins. Some of these are expressed in a cell-specific manner. Their detection can therefore have a high diagnostic value in neoplastic and inflammatory processes. Some of the proteins included in the CD-classification have receptor or signaling functions, while others carry out enzymatic activities or are part of intercellular communication.

Classification
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CD1: Family of several human glycoproteins expressed on the surface of various antigen-presenting cells They belong to the so-called MHC-like glycoproteins and occur in humans in 5 different isoforms: CD1a-CD1e.

CD1a: marker for: thymocytes, Langerhans cells, interdigitating cells, B-cell subtypes, dendritic cells, cortical thymocytes, thymomas, T-cell lymphomas, lymphoblasts.

CD1c: Like the other members of the CD1 family, it belongs to the MHC-I-like proteins. Is expressed mainly by cortical thymocytes.

CD2: surface marker for T lymphocytes, NK lymphocytes

CD3: surface marker for T-lymphocytes, the CD3 complex is associated with the T-cell receptor (see below lymphoma, cutaneous T-cell lymphoma)

CD4: Labeled T-helper cells, monocytes, receptor for HIV (see below lymphoma, cutaneous T-cell lymphoma)

CD5: Labeled T lymphocytes, B cell subgroup

CD6

: CD6 is a marker for T cells, thymocytes and CLL B cells and binds CD166. CD6 is responsible for the activation, co-stimulation, adhesion and differentiation of these lymphocytic cells

CD7: T lymphocytes, NK lymphocytes, (the pattern CD3+, CD4+, CD7- = pathognomic for Sézary cells)

CD8: cytotoxic T lymphocytes, NK cell subgroup

CD10: CD10 is a membrane associated neutral endopeptidase. CD10 is useful in the diagnosis of lymphoblastic leukemia, follicular lymphoma and Burkitt's lymphoma.

CD11a: lymphocytes, (LFA-1), binding partner for CD54 (ICAM-1)

CD11b: Monocytes, granulocytes, macrophages and NK cells

CD13: Myeloid cells, (acute myeloid leukemia)

CD14: Marker for: monocytes, macrophages, Langerhans cells, dendritic cells; also B cells and neutrophils. CD14 is a surface membrane protein and acts as an affinity receptor for the complex of lipopolysaccharides (LPS) and LPS binding protein (LBP).

CD15: CD15 is a marker for: granulocytes, monocytes, myeloid cells, dendritic reticulum cells, Hodgkin and Sternberg Reed cells and carcinomas in the epithelia (sebaceous glands, sweat glands).CD15 acts as a ligand for selectins and is involved in cell adhesion through a direct Lewis X-Lewis X interaction.

CD16: Marker for: NK cells, macrophages, neutrophil granulocytes. CD 16 designates two surface proteins from the group of Fc receptors.

CD18: Marker for: leukocytes, activated thrombocytes

CD19: marker for: B cells, is not expressed on plasma cells, including follicular dendritic cells. CD19 is involved in B-cell development, activation and differentiation. Expressed by all neoplastic B cells.

CD20: B-lymphocytes, isolated T-lymphocytes (see also marginal zone lymphoma); CD 20 antibodies are successfully used in B-cell lymphomas, in various types of cancer and in the treatment of cancer. Autoimmune diseases (see pemphigus vulgaris) and malignant melanoma.

CD21

: marker for: B-cells, follicular dendritic cells. CD21 is involved in B-cell activation and proliferation. Belongs to the family of complement regulatory proteins. CD21 is a receptor for the Epstein-Barr Virus (EBV). Marks the germinal centers of reactive lymph follicles.

CD22:

Marker for: mature B cells. CD22 binds CD75 and sialon conjugates

CD23: marker for: B cells, monocytes, follicular dendritic cells, eosinophil granulocytes.CD23 is associated with MHC class II antigen. Low affinity receptor for IgE. Expression is upregulated after mitogen or antigen activation. The antibody marks germinal centres of reactive follicles (see alsoCD 35).

CD24

: Marker for: B cells, granulocytes

CD25: marker for: activated T and B cells, regulatory T cells, activated monocytes, macrophages. CD25 is the low affinity receptor for IL-2 (IL-2Rα). In combination with CD122 and CD132, CD25 forms the high affinity IL-2R complex.

CD26: Marker for: activated T and B cells, macrophages. Dipeptidylpeptidase IV (ectoenzyme). The marker combination CD3+/CD4+/CD26- is pathognomic for Sézary cells in peripheral blood.

CD27: marker for: activated B cells, T cells, NK cells, thymocytes. CD27 belongs to the tumor necrosis factor receptors (TNFR). Predominantly expressed by naive helper cells (CD45RA+/CD45RO-).

CD28: Protein belonging to the immunoglobulin superfamily. CD28 is homologous to CTLA-4 (= CD152) and is mainly expressed on activated CD4+T lymphocytes and CD8+ T lymphocytes; it inhibits T cell proliferation.

CD29

: marker for: leukocytes. CD29 is an integrin-beta1 subunit (see integrins below) and binds with alpha and alpha6 integrins(CD49).

CD30: Activated lymphocytes, Sternberg-Reed cells, Ki-1 lymphoma cells in histology (see below lymphoma, cutaneous T-cell lymphoma; lymphomatoid papulose)

CD31: Transmembrane glycoprotein, also known as platelet-endothelium cell adhesion molecule, platelet endothelial cell adhesion molecule" PECAM-1 CD31 plays a role in thrombosis and angiogenesis (v. Willebrand factor). It is expressed strongly in endothelial cells and weakly in megakaryocytes, platelets and occasionally in plasma cells.

CD32:

Marker for: granulocytes, monocytes, eosinophilic leukocytes, B cells. CD32 is an Fc-receptor and active in phagocytosis.

CD33: marker for: monocytes, myeloid progenitor cells. CD33 binds sialon conjugates by adhesion

CD34: Member of the family of transmembrane sialomucine proteins expressed by endothelial cells, hematopoietic stem cells and immature fibroblasts(Dermatofibrosarcoma protuberans)

CD35

: Immunohistological marker (formalin-fixed, paraffin-embedded material) for follicular dendritic cells, B cells and monocytes in peripheral blood only. CD35 marks follicular germinal centres.

CD36: Marker for: platelets, monocytes, endothelial cells. CD36 is a platelet adhesion molecule involved in the recognition and phagocytosis of apoptotic cells.

CD37: Marker for: mature B and T cells, myeloid cells.

CD38: activated T and B lymphocytes, plasma cells, HIV monitoring (CD8/CD38)

CD39

: Marker for: macrophages, B cells, basal epithelial cells, dendritic cells

CD40: Marker for: B cells, interdigitating cells, fibroblasts, macrophages, follicular dendritic cells. The binding of CD40 to CD154 on T-cells enables growth and differentiation of B-cells.

CD41: marker for: platelets, megakaryocytes. CD41 binds with CD61 to form GPIIb. In addition, CD41 binds fibrinogen, fibronectin, thrombospondin and von Willebrand factor.

CD43: Acronym for cluster of differentiation 43: CD43 is a marker for T cells (no B cells, except immature activated B cells); furthermore myeloid cells (granulocytes, monocytes). The binding of CD40 and CD154 to T-cells enables the growth and differentiation of B-cells. CD43 is not a specific T-cell marker, but the coexpression of CD43/CD5 with CD20 is considered an important indication of the presence of low-malignant B-cell lymphoma (e.g. B-CLL).

CD44: Marker for: erythrocytes, leucocytes. CD44 binds hyaluronic acid and mediates the adhesion of leukocytes

CD45: all leucocytes

CD45RA: B lymphocytes, NK lymphocytes, naive T lymphocytes

CD45RB

: CD45RB labels B cells, T cells, macrophages, granulocytes

CD45RO: Memory T lymphocytes, monocytes, granulocytes

CD54: Adhesion molecule (ICAM-1), ligand for LFA-1 (CD11a)

CD56: NK lymphocytes, higher antigen density when activated; (Note: CD 56 = identical to the neuronal adhesion molecule N-CAM). CD56 is expressed in myeloma cells, myeloid leukaemia, neuroendocrine tumors, adult neuroblastomas, NK/T-cell lymphomas, small cell lung carcinoma, Ewing sarcoma.

CD63: (also LIMP-1 -lysosome integral membrane protein 1- or LAMP-3 lysosomal associated membrane glycoprotein 3). Membrane glycoprotein which is produced by various membrane glycoprotein expressed by various cells of the hematopoietic system both on the cell surface and inside the cells. In basophilic granulocytes, CD63 is mainly anchored in the granula membranes and is only expressed on the membrane surface when the cell is activated during the fusion between granule and cell membrane. Thus, CD63 can be used as an activation marker for basophils (see Basophile Activation Test) (see also CD 203c).

CD68: Transmembrane glycoprotein located mainly in lysosomes and belonging to the family of LAMP glycoproteins involved in lysosomal transport or endocytosis. The antibody (PG-M1) is suitable for labelling tissue macrophages. It does not stain myeloid progenitor cells, granulocytes and dendritic cells.

CD69: activated T, B, NK lymphocytes

CD79a: Pan B cell marker (often more sensitive than CD20). Indication: B-cell lymphomas and leukemias. Also suitable for the detection of B-precursor cells (lymphoblastic leukemias, see also lymphoma, cutaneous B-cell lymphoma, plasmocytoma).

CD95: apoptosis receptor (TNF family; FAS), elevated in viral infections (AIDS) CD95 is one of 6 identified receptors that can directly induce apoptosis. CD95 is constitutively expressed in many tissues. The expression of the CD95 ligand (CD95L) is restricted to a few cells, such as activated T cells, macrophages. CD95L is strictly regulated and is not expressed on resting T cells. After stimulation of the T cell receptor (TCR) / CD3 complex by antigen, it is strongly upregulated.

CD103: Integrin; CD103 is produced by intraepithelial T lymphocytes and by regulatory T cells (Tregs). Clinically-diagnostically, it serves as a marker for tumor cells of hairy cell leukemia.

CD117: Kit (also c-Kit) is mainly found in hematopoietic stem cells but also in multipotent and myeloid progenitor cells. CD117 can be activated by its ligand SCF (stem cell factor). Kit is essential for the proliferation and differentiation of stem cells (hematopoiesis and angiogenesis).

CD141: Thrombomodulin is a protein that acts as a transmembrane receptor for thrombin in the endothelial cell. Thombomodulin regulates coagulation within the uninjured vessel.

CD152: CTLA-4 protein which belongs to the immunoglobulin superfamily. CTLA-4 is homologous to CD28 and is mainly expressed on activated CD4+T lymphocytes and CD8+ T lymphocytes and inhibits T-cell proliferation by reducing the expression of the IL-2 receptor, thus also reducing IL-2 production. In various approaches, blocking CTLA-4 by monoclonal antibodies (see → Ipilimumab) could inhibit tumor growth (see also melanoma, malignant).

CD191: CD191 denotes the C-C chemokine receptor type 1(CCR1). CD191 is a member of the beta-chemokine receptor family, which belongs to the G-protein-coupled transmembrane chemokine receptors. The CCR1 receptor is expressed by different cells in different densities, such as neutrophil and eosinophil granulocytes, monocytes, T cells, osteoclasts.

CD203c Phenotypic marker of basophilic granulocytes (also referred to as PD-Iβ, PDNP3 and gp130RB13-6).

CD203c = multifunctional ectoenzyme, belongs as ENPP3 to the group of ecto-nucleotide pyrophosphatases - phosphodiesterases (ENPPs). They cleave phosphodiester and phosphosulfate bonds of nucleotides and hydrolyze the nucleotides. CD203c is detected in uterus, prostate, glioma cells as well as in colon and bile duct carcinomas. In the hematopoietic system, CD203c is expressed exclusively by mature basophils and mast cells and their CD34+ precursor population. Basophil activation results in a significant increase in CD203c expression on the cell surface, which can be measured by means of suitable AK (see basophil activation test; see also CD 63).

CD206= mannose receptor, a C-type lectin expressed primarily by macrophages and immature dendritic cells. CD206 is also expressed on human keratinocytes and dermal fibroblasts.

CD207 = synonymous with Langerin. The protein is expressed by Langerhans cells, CD103-positive dendritic cells of skin and CD8-positive dendritic cells of spleen.

CD280 = mannose receptor, a C-type lectin primarily expressed by macrophages and immature dendritic cells.

General information
This section has been translated automatically.

CD nomenclature (CD=acronym for "cluster of differentiation"). The CD antigens are also used for immunohistological questions and play an important role in the differentiation of inflammatory and neoplastic infiltrates. See below immunohistology.

Literature
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  1. Oba J et al (2011) Expression of CD10 predicts tumor progression and unfavorable prognosis in malignant melanoma. J Am Acad Dermatol 65:1152-1160

Tables
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Immunohistologically applicable markers for B and T cells

Antigen

Clone

Remarks

Formalin/Paraffin

Frozen section

B-cells

CD19

HD37

B-cell specific

-

+

CD20

L26

B-cell specific

+

+

CD21

1F8

+

+

CD22

4KB128

-

+

CD23

MHM6

-/+

+

CD45RA

4KB5

also T-cells

+

+

CDw75

LN1

+

+

CD79

JCB117

B-cell specific

+

+

CD10

SS2/36

CALLA

-

+

Kappa

polyclonal

light chains

-

+

Lambda

polyclonal

light chains

-

+

unknown

VS38c

Plasma cell markers

+

+

T-cells

CD2

MT910

-

+

CD3

polyclonal

T-cell specific

+

+

CD4

MT310

-

+

CD5

DK23

-

+

CD7

DK24

-

+

CD8

C8/144B

+

+

CD26

-

+

CD43

DF-T1

+

+

CD45R0

UCHL1

+

+

Overview of immunohistologically applicable antibodies

CD antigen

Cells, structure, annotations and applications

CD1a

cells: thymocytes, Langerhans cells, interdigitating cells, B-cell subtypes

Structure: Membrane glycoprotein (49 kDa)

Note: CD1a is expressed in association with beta 2-microglobulin. CD1a antibodies are helpful in assessing the differentiation of T cells in the thymus

Application: FACS, IHC (P) (G), IF

CD2

Cells: T cells, NK lymphocytes, thymocytes

Structure: Transmembrane protein (50 kDa)

Note: CD2 is a receptor for CD58 (LFA-3) and mediates interactions between T cells and antigen-presenting cells. Very early T-cell antigen.

Application: FACS, IHC (P) (G), IF

CD3

Cells: T-cells, thymocytes

Structure: Complex of 5 unvarying polypeptide chains (25-28, 21, 20, 16 and 22 kDa)

Note: CD3 is part of a larger complex that includes the T cell receptor (TCR). T-cell activation by a foreign antigen is presented to the TCR by the MHC.

Application: FACS, IHC (P) (G), IF

CD4

cells: T-helper cells, thymocytes, monocytes, macrophages

Structure: Transmembrane protein (59 kDa)

Note: Binds to MHC class II molecules and is a co-receptor for MHC class II antigen-induced activation. On thymocytes often co-expressed with CD8. CD4+ lymphocytes are involved in the synthesis of immunoglobulins by B cells. CD4 is a receptor for gp120 (envelope protein of HIV-1).

Application: FACS, IHC (P) (G), IF

CD5

Cells: T cells, thymocytes, subunit of B cells

Structure: Transmembrane protein (67 kDa)

Note: CD5 is a ligand for CD72 expressed on B cells

Application: FACS, IHC (P) (G), IF

CD7

Cells: T cells, NK cells, monocytes, pre-B cells

Structure: Transmembrane protein (40 kDa)

Note: CD7 (gp40), belonging to the immunoglobulin superfamily, is also expressed in acute lymphocytic leukemia (T-cell ALL). Very early T-cell antigen. The marker combination CD3+CD4+CD7- is pathognomic for Sézary cells in peripheral blood.

Application: FACS, IHC (G), IF

CD8

cells: cytotoxic T cells, NK cells, thymocytes

Structure: Transmembrane protein, disulfide linked dimer (32-34 kDa)

Note: The CD8 antigen acts with the TCR as a co-receptor for MHC class I antigen recognition. On thymocytes often co-expressed with CD4.

Application: FACS, IHC (P) (G), IF

CD9

Cells: Platelets, monocytes, endothelial cells, eosinophils, basophils, activated T cells

Structure: Transmembrane protein (24 kDa)

Note: The CD9 antigen is associated with integrins and HLA-DR, therefore probably functions in adhesion and signal transduction.

Application: FACS, IHC (P) (G), IF

CD10

cells: B and T progenitor cells, granulocytes, various cells of epithelial origin

Structure: Transmembrane protein (100 kDa)

Note: Membrane-associated neutral endopeptidase. Important as an acute lymphoblastic leukemia antigen (Common acute lymphatic leukemia antigen [CALLA]).

Application: FACS, IHC (P) (G), IF

CD11a

cells: B cells, T cells, monocytes, activated platelets, granulocytes, macrophages

Structure: Transmembrane glycoprotein (170 kDa)

Note: Belongs to the integrins associated with a subunit of CD18; LFA-1 (CD11a/CD18) promotes the adhesion of leukocytes to the vascular endothelium. Important ligands of LFA-1 are the intercellular adhesion molecules (ICAM?s)

Application: FACS, IHC (P) (G), IF

CD14

Cells: monocytes, macrophages, Langerhans cells, dendritic cells; also B cells and neutrophils

Structure: Surface membrane protein (53-55 kDa)

Note: CD14 acts as an affinity receptor for the complex of lipopolysaccharides (LPS) and LPS binding protein (LBP).

Application: FACS, IHC (P) (G), IF

CD15

cells: granulocytes, monocytes

Structure: Carbohydrate antigen

Note: CD15 serves as a ligand for selectins and is involved in cell adhesion through a direct Lewis X-Lewis X interaction. Expressed on leukemic cells of myelo-monocytic origin, in rare cases also in ALL.

Application: FACS, IHC (P) (G), IF

CD18

Cells: Leukocytes, activated thrombocytes

Structure: Integrin-ß2 chain (95 kDa)

Note: see under CD11a

Application: FACS, IHC (P) (G), IF

CD19

cells: B cells, not on plasma cells, also follicular dendritic cells

Structure: Membrane glycoprotein (95 kDa)

Note: CD19 is involved in B-cell development, activation and differentiation. Expressed by all neoplastic B cells.

Application: FACS, IHC (P) (G), IF

CD20

cells: B cells, not on plasma cells, in low density also on T cells

Structure: Membrane protein (isoforms 33, 35, 37 kDa)

Note: CD20 is involved in B cell development, activation and differentiation. Expressed in ALL, B-cell CLL, hairy cell leukemia and Burkitt's lymphoma cells.

Application: FACS, IHC (P), IF

CD21

Cells: B cells

Structure: Transmembrane glycoprotein (145 kDa)

Note: CD21 is involved in B cell activation and proliferation. Belongs to the family of complement regulatory proteins. CD21 is a receptor for Epstein-Barr Virus (EBV)

Application: FACS, IHC (P) (G), IF

CD23

cells: B cells, monocytes, follicular dendritic cells, eosinophils

Structure: Transmembrane glycoprotein (45 kDa)

Note: CD23 is associated with MHC class II antigen. Low affinity receptor for IgE. Expression is upregulated after mitogen or antigen activation.

Application: FACS, IHC (P) (G), IF

CD25

cells: activated T and B cells, regulatory T cells, activated monocytes, macrophages

Structure: Glycoprotein (55 kDa)

Note: CD25 is the low affinity receptor for IL-2 (IL-2Rα). In combination with CD122 and CD132, CD25 forms the high affinity IL-2R complex.

Application: FACS, IHC (P) (G), IF

CD26

cells: activated T and B cells, macrophages

Structure: Glycoprotein (110 kDa)

Note: Dipeptidyl peptidase IV (ectoenzyme). The marker combination CD3+/CD4+/CD26- is pathognomic for Sézary cells in peripheral blood.

Application: FACS, IHC (G), IF

CD27

cells: activated B cells, T cells, NK cells, thymocytes

Structure: Transmembrane glycoprotein (55 kDa)

Note: CD27 belongs to the tumor necrosis factor receptors (TNFR). Mainly expressed by naive helper cells (CD45RA+/CD45RO-)

Application: FACS, IHC (G), IF

CD28

Cells: T cells, plasma cells, thymocytes

Structure: Membrane protein (44 kDa)

Note: CD28 is involved in the adhesion of T cells to B cells. CD4+ cells express the antigen more frequently than CD8+ cells.

Application: FACS, IHC (G), IF

CD30

cells: activated T and B cells, Hodgkin and Reed-Sternberg cells, anaplastic large cell lymphoma cells

Structure: Transmembrane cytokine receptor (120 kDa)

Note: CD30 (Ki-1 antigen) belongs to the tumor necrosis factors. The antigen plays a role in regulating the function, differentiation and/or proliferation of normal lymphoid cells.

Application: FACS, IHC (P) (G), IF

CD31

cells: stem cells of the myeloid series, platelets

Structure: Membrane glycoprotein (130 kDa)

Note: Synonym: Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1). CD31 is involved in the migration of leukocytes through the endothelium. Also involved in platelet activation and aggregation.

Application: FACS, IHC (P) (G), IF

CD34

Cells: Haematopoietic progenitor cells, pluripotent stem cells, capillary endothelial cells, bone marrow stroma cells

Structure: Transmembrane glycoprotein (110 kDa)

Note: The highest expressions of CD34 are found on primitive stem cells. They gradually disappear during differentiation and maturation of the progenitor cells. About 60% of all acute B-lymphoid leukemias and 40% of acute myeloid leukemias (AML) and up to 5% of acute T-lymphoid leukemias express CD34.

Application: FACS, IHC (P) (G), IF

CD38

cells: T and B cells, monocytes, plasma cells, thymocytes

Structure: integral membrane glycoprotein (45 kDa)

Note: CD38 expressions are dependent on differentiation and activation states of the cells. CD38 is often used as a marker for T and B cell activation. HIV monitoring (CD8/CD38)

Application: FACS, IHC (G), IF

CD40

cells: B cells, interdigitating cells, fibroblasts, macrophages, follicular dendritic cells

Structure: Phosphoglycoprotein (44-48 kDa)

Note: Binding of CD40 to CD154 on T-cells enables growth and differentiation of B-cells.

Application: FACS, IHC (P) (G), IF

CD43

Cells: Leukocytes (resting B cells weak, activated B cells strong expression), platelets

Structure: Membrane glycoprotein (95-135 kDa)

Note: Binding of CD40 to CD154 on T cells allows growth and differentiation of B cells.

Application: FACS, IHC (P) (G), IF

CD45

Cells: all leukocytes: lymphocytes, eosinophils, monocytes, basophils, neutrophils (in decreasing expression density)

Structure: integral membrane protein (5 isoforms 180-220 kDa)

Note: Leucocyte common antigen (LCA). CD45 is a major component of the lymphocyte membrane. CD45 antibody helpful in neoplastic B and T cells of non-Hodgkin lymphomas and leukemias.

Application: FACS, IHC (P) (G), IF

CD45RA

Cells: B cells, naive/resting CD4+/ CD8+ cells, monocytes

Structure: integral membrane protein (CD45 isoform, 220 kDa)

Note: CD45RA antibodies can be used for functional differentiation of CD4+ and CD8+ cells.

Application: FACS, IHC (P) (G), IF

CD45R0

Cells: Memory CD4+ cells, activated T cells, granulocytes, monocytes

Structure: integral membrane protein (CD45 isoform, 180 kDa)

Note: CD45R0 antibodies can be used for functional differentiation of CD4+ and CD8+ cells.

Application: FACS, IHC (P) (G)

CD50

Cells: all leucocytes

Structure: Membrane protein (120-130 kDa)

Note: Also known as Inter-Cellular Adhesion Molecule-3 (ICAM-3), it interacts with LFA-1 in cellular adhesion processes.

Application: FACS, IHC (P)

CD54

cells: endothelial cells, resting and activated lymphocytes and monocytes

Structure: Transmembrane glycoprotein (90 kDa)

Note: Also known as Inter-Cellular Adhesion Molecule-1 (ICAM-1). CD54 is the ligand for CD11a (LFA-1). CD54 mediates the adhesion of T cells to antigen-presenting cells (APCs) and from T cell to T cell and T cell to B cell interactions. CD54 is also a receptor for rhinoviruses.

Application: FACS, IHC (P) (G), IF

CD56

cells: NK lymphocytes

Structure: Membrane glycoprotein (140 kDa)

Note: CD56 is also expressed by large granular lymphocytes. The antigen is also present on neuronal tissues and some tumours. When activated, a higher antigen density is found.

Application: FACS, IHC (P) (G), IF

CD58

Cells: Leukocytes, erythrocytes, endothelial cells

Structure: Transmembrane glycoprotein (65-70 kDa)

Note: Also known as lymphocyte-function associated antigen 3 (LFA-3) and interacting with CD2. CD58 is involved in the adhesion of killer cells and target cells as well as APCs and T cells.

Application: FACS, IF

CD62L

cells: B and T cells, monocytes, neutrophils, eosinophils, NK cells

Structure: Glycoprotein (75 kDa)

Note: Synonym: LECAM-1, L-selectin. CD 62L is involved in the ?homing? of lymphocytes to the peripheral lymph nodes.

Application: FACS, IHC (G), IF

CD63

Cells: Granulocytes, monocytes, thrombocytes, melanoma cells

Structure: Glycoprotein (40-60 kDa)

Note: CD63 is an intracellular lysosomal granulaprotein which is translocated to the cell surface after activation of granulocytes/endothelial cells (activation marker). CD63 is identical to ME491, an antigen expressed by melanoma cells and associated with early stages of tumor progression.

Application: FACS, IHC (P)

CD69

cells: leucocytes, erythrocytes, endothelial cells

Structure: Transmembrane glycoprotein (65-70 kDa)

Note: Also known as lymphocyte-function associated antigen 3 (LFA-3) and interacting with CD2. CD69 is involved in the adhesion of killer cells and target cells as well as APCs and T cells.

Application: FACS, IHC (G), IF

CD95

Cells: CD4+, CD8+ T cells, B cells, variable also on monocytes and granulocytes, tissue

Structure: Transmembrane glycoprotein (40-50 kDa)

Note Synonyms: Fas, APO-1, CD95 belongs to the group of tumor necrosis factors and is mainly responsible for apoptosis (programmed cell death). Ligand is CD 178, CD95 is upregulated when cells are activated.

Application: FACS, IHC (P) (G)

CD99

Is expressed on most human tissue. Cortical lymphocytes, almost all hematopoietic cells (except granulocytes). Furthermore in a wide range of tumors (Ewing's sarcoma, T-cell lyphoma, calcifying aponeurotic fibroma)

CD126

cells: activated B cells, CD4+ cells, monocytes, epithelial cells

Structure: integral membrane protein (80 kDa)

Note: In combination with CD 130, CD 126 forms the high-affinity IL-6 receptor.

Application: IHC (G)

CD127

Cells: T cells, monocytes, precursor B cells

Structure: Membrane glycoprotein (75-80 kDa)

Note: Synonym: IL-7 receptor-alpha. The receptor expression is downregulated after activation. CD127 plays an important role in the proliferation and differentiation of T cells.

Application: FACS

CD130

cells: activated B cells, EBV-transformed B cells (high expression), many cell types (low expression)

Structure: Transmembrane glycoprotein (130 kDa)

Note: In combination with CD 126, CD 130 forms the high-affinity IL-6 receptor.

Application: FACS

CD134

cells: activated CD4+ cells, virally stimulated CD4+ cells (high expression)

Structure: Transmembrane glycoprotein (35 kDa)

Note: CD 134 belongs to the tumor necrosis factors. CD 134 is jointly responsible for the clonal expansion of CD4+ cells and for the formation of memory T cells. CD 134 also appears to be involved in T-cell adhesion processes.

Application: FACS, IHC (G)

CD154

cells: activated CD4+ cells, also basophils, mast cells

Structure: Transmembra protein (32 kDa)

Note: CD 154 belongs to the tumor necrosis factors. CD 154 takes over regulatory tasks in T- and B-cell function.

Application: FACS

CD178

cells: activated T cells, NK cells

Structure: Membrane protein (40 kDa)

Note: Synonym: FAS Ligand (FasL). CD 178 belongs to the tumor necrosis factors. The binding of CD 178 to its receptor CD 95 induces apoptosis.

Application: FACS, IHC (P)

CD184

cells: hematopoietic cells, dendritic cells

Structure: Transmembrane surface receptor

Note: Chemokine receptor. Synonyms: CXCR4, Fusin. Natural ligand of SDF-1. CD 184 is an essential cofactor for the fusion and entry of T-cell lymphotropic HIV-1 viruses in CD4+ T-cells.

Application: FACS, IHC (P)

CD191

CD191 denotes the C-C chemokine receptor type 1(CCR1).

Cells: The CCR1 receptor is expressed by different cells at different densities, such as neutrophil and eosinophil granulocytes, monocytes, T cells, osteoclasts.

CD195

Cells: T-cells, monocytes

Structure: Glycoprotein (45 kDa)

Note: Chemokine receptor. Synonym: CCR5. Important receptor for RANTES and cofactor for macrophage-trophic HIV strains.

Application: FACS, IHC

CD209

cells: dendritic cells

Structure: Transmembrane lectin (44 kDa)

Note: Synonym: dendritic cell-specific-intracellular adhesion molecule 3 (ICAM-3)-grabbing non-integrin (DC-SIGN). CD209 plays a key role as receptor for various ligands such as ICAM-2, ICAM-3, HIV-1 gp120.

Application: FACS, IHC (G)

Authors

Last updated on: 29.10.2020