Author: Prof. Dr. med. Peter Altmeyer

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Last updated on: 29.10.2020

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Method for the detection of antigens in tissue by means of antibodies coupled to fluorochromes (fluorescein, phycoerythrin, Texas Red) (DIF = direct IF) or by the sandwich method i.e. with unlabelled specific antibodies whose binding is detected with a second labelled antibody (IIF = indirect IF).

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  • DIF is an indispensable detection of autoantibodies in blistering autoimmune diseases. It is carried out on frozen sections of perilesional or even heart-free healthy skin. In pemphigus diseases the blistering is intraepithelial, in other bullous autoimmune dermatoses subepithelial (e.g. pemphigoids). A further differentiation of the antigens in subepidermal cleft formation is possible by means of the salt-split skin examination.
  • IIF is a detection method for the characterization of circulating autoantibodies (e.g. desmogleins from the protein family of cadherins in pemphigus vulgaris) on a suitable substrate (e.g. monkey esophagus or rat bladder). S.u. pemphigus and pemphigoid.


Last updated on: 29.10.2020