Testicular biopsy

Removal of a 1-2 mm large testicular parenchyma piece under local anesthesia (without adrenaline addition!) and subsequent tissue fixation in Bouin's solution (total aqueous picric acid 15.0; formaldehyde 40% 5.0; glacial acetic acid 1.0) or glutaraldehyde 5.5% in phosphate buffer (for semi-thin section technique) for histological examination.

Always biopsy both testicles. Stretch the scrotal skin tightly over the testes. 2 cm long skin incision over the lateral third of the testis, loosen the tunica vaginalis from the scrotal skin with dissecting scissors and expose it, split the tunica vaginalis, insert a self-retaining eyelid spreader, incision of the tunica albuginea, incision max. 0.5 cm; testicular tissue swells spontaneously above the level of the testicular capsule, can be cut off with small curved scissors. Subsequently closure in layers; first tunica albuginea (thread thickness 5-0), then tunica vaginalis (thread thickness 4-0) and scrotal skin with atraumatic absorbable suture material. Cave! Injury of the epididymis or spermatic cord!