Annexin V Antigentest

ELISA test for the quantitative determination of Annexin V in human plasma or in any other biological medium. Recombinant and native Annexin are both measured by the ZYMUTEST Annexin V assay. This test can also be used for quality control of platelet or blood cell concentrates.

Annexin V is a 35 kD Ca2+-dependent protein, a member of a family of Ca2 +-binding proteins. There are now over 100 annexins sequenced in 45 species, 12 of which are in humans. Annexin V is present in many tissues and mainly in endothelial cells and placenta. At lower concentrations, annexin V is present in platelets and at higher concentrations in red blood cells and leukocytes. It binds Ca2+-dependently to anionic phospholipids (phosphatidylserine) and activated cell membranes. Furthermore, Annexin V also inhibits the procoagulant and proinflammatory activity of apoptotic cells. Annexin V serves for the early detection of apoptosis.

The concentration of annexin V in human plasma is usually less than 10 ng/ml.

1. Miwa N et al.(2008) S100-annexin complexes--biology of conditional association. FEBS J 275:4945-4955.
2. Rescher U et al. (2004) Annexins--unique membrane binding proteins with diverse functions. J Cell Sci 117:2631-2639.