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DefinitionThis section has been translated automatically.
Staining method for staining mycobacteria.
General informationThis section has been translated automatically.
Implementation:
- Dry and heat fix preparations
- Cover with carbolic fox solution
- Heat the slides with a Bunsen flame from below until steam is formed ( do not boil!)
- let cool down
- Repeat heating and cooling 2x
- Rinsing with water
- Decolorize with HCL alcohol until no more color clouds come off
- Rinsing with water
- Counter staining with malachite green 1 min.
- Rinsing with water
Result: Acid-resistant rods pink to red, other germs and cells green.
Prof. Dr. med. Peter Altmeyer