Cryopreservation

Author: Prof. Dr. med. Peter Altmeyer

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Last updated on: 29.10.2020

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Definition
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After adding a protective medium (glycerin/egg yolk preparation) to the native jelly, the latter is portioned and placed in liquid nitrogen (-196 °C) in special freezing processes to quickly overcome the crystallisation point and, if necessary, thawed in a rapid process. The storage period ranges from several months to years, whereby the quality of the cryosperm decreases with increasing storage time. Compared to native sperm, thawed cryosperm is of significantly reduced quality with an average loss of motility and vitality of 50%, so that the achievable pregnancy rate with cryosperm is also considerably reduced.

Indication
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Men with testicular tumors, lymphomas and other malignancies before initiation of cytostatic therapy or radiotherapy for homologous insemination. Sperm donors for heterologous insemination.

Literature
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  1. Köhn FM et al. (1988) Kryospermabank Munich - interim balance 1974-1986. dermatologist 39: 91-96

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Last updated on: 29.10.2020