Complement fixation reaction

Synonym(s)

KBR

Definition
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Detection system of an antigen-antibody reaction under complement consumption.

Indication
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Field of application: Diagnostics of viral infections, Wassermann complement binding reaction, Sabin-Feldman test.

Implementation
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A known antigen and a well-dosed amount of complement is added to an unknown serum sample. An antigen-antibody reaction takes place in the serum, i.e. if antibodies against the known antigen are present, the complement is consumed. After the addition of an indicator system consisting of sheep erythrocytes and rabbit antibodies directed against them, if an antigen-antibody reaction has taken place and complement has thus been consumed, erythrocyte lysis does not occur.