DefinitionThis section has been translated automatically.
The allergogram serves to identify the main, intermediate and minor allergens. For this purpose, the proteins of an extract are separated electrophoretically. They are then transferred to a nitrocellulose membrane and incubated with allergenic serum. During this process the IgE antibodies bind to the allergenic proteins. This allergen-IgE complex is made visible by adding radio-isotope or enzyme-labelled anti-IgE antibodies. An extract is tested with at least 20 patient sera. Those proteins that are recognized by more than 50% of allergy sufferers are considered the main allergen.