Mifc method
Synonym(s)
Merthiolate-iodine-formaldehyde concentration process
DefinitionThis section has been translated automatically.
Enrichment system on sedimentation basis (centrifugation) for the examination of stool samples in parasitology, e.g. for worm eggs
General informationThis section has been translated automatically.
- Parasite stages are permanently fixed in MF solution. Coarse excrement components are filtered off, the sample is degreased by ether and parasite stages are sedimented by centrifugation.
- MF solution: Thimerosal 1:1000 or merthiolate tincture 200 ml; formaldehyde 37-40% 25 ml; glycerine 5 ml; distilled water. 250 ml.
IndicationThis section has been translated automatically.
Nematode, cestoda and trematode eggs; in case of increased excretion also lungworm and Strongyloides larvae; faecal stages of intestinal protozoa (coccidia, giardia, amoeba, etc.).
ImplementationThis section has been translated automatically.
- Place a bean/hazelnut-sized, body-warm faeces sample in a test tube containing approx. 10 ml MF solution, close the tube and shake vigorously.
- Shake up the fixed faeces suspension and strain about half of it through gauze over funnels into centrifuge tubes.
- Add approx. 2 ml ether, close tubes with rubber stoppers and shake carefully; remove stoppers.
- Centrifuge at 1000-1500 rpm.
- Decant the upper 3 layers, transfer 1-2 drops of the sediment with a pasteur pipette onto slides, add 1 drop of Lugol's solution, place cover glass.
- Examine initially at 100x, then at 400x magnification with a transmitted light microscope.
Remember! Dispose of used liquids (mercury content) as hazardous waste.
Note(s)This section has been translated automatically.
Remember! The MIFC technique has been recommended by the parasitological commission of the BGA as the method of choice.