HiSeq 4000 (Hseq stands for High-Throughput Sequencing) is a high-throughput DNA sequencing device from Illumina - more precisely, a platform for Next-Generation Sequencing (NGS), i.e. for the parallel sequencing of millions of DNA fragments. The process is primarily used in research laboratories and large genome centers.
HiSeq 4000
DefinitionThis section has been translated automatically.
General informationThis section has been translated automatically.
The HiSeq 4000 is an automated sequencing system based on Illumina's Sequencing-by-Synthesis (SBS) technology that can generate extremely large amounts of DNA sequence data in a short time. It is suitable for: whole genome sequencing, exome sequencing, RNA sequencing (RNA-Seq), epigenomics (e.g. metylation analysis).
Basic principle:
- DNA is fragmented and provided with adapters
- The fragments are immobilized on a so-called flow cell
- There they are amplified into clusters by bridge amplification
- This is followed by cyclic sequencing with fluorescently labeled nucleotides
- The device reads millions to billions of DNA fragments in parallel. In a single sequencing run, analyses of up to 1.5 terabases per run are possible.
- The runtime is generally 3-4 days per sequencing run.
- The method is used in genomics, transcriptomics and epigenomics.